Volume 25, Issue 3 (7-2025)
ijdld 2025, 25(3): 195-205 |
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Bagheri A, Morady M, Malekipooya M, Abedi B. The Effect of a Period of Endurance Training Along with Electrical Stimulation on the Gene Expression of Some Sex Markers in the Testicular Tissue of Fasted Obese Rats. ijdld 2025; 25 (3) :195-205
URL: http://ijdld.tums.ac.ir/article-1-6394-en.html
URL: http://ijdld.tums.ac.ir/article-1-6394-en.html
1- Department of Exercise Physiology, Faculty of Humanities, Mahallat Branch, Islamic Azad University, Mahallat, Iran
2- Department of Exercise Physiology, Faculty of Humanities, Arak Branch, Islamic Azad University, Arak, Iran
3- Department of Exercise Physiology, Faculty of Humanities, Arak Branch, Islamic Azad University, Arak, Iran ,Mo.malekipooya@iau.ac.ir
4- Department of Physical Education, North Tehran Branch, Islamic Azad University, Tehran, Iran
2- Department of Exercise Physiology, Faculty of Humanities, Arak Branch, Islamic Azad University, Arak, Iran
3- Department of Exercise Physiology, Faculty of Humanities, Arak Branch, Islamic Azad University, Arak, Iran ,
4- Department of Physical Education, North Tehran Branch, Islamic Azad University, Tehran, Iran
Abstract: (2068 Views)
Background: Obesity is a multifactorial disease whose incidence and burden on societies around the world is increasing. Sexual performance in these patients is an important aspect that is often ignored. Therefore, the aim of the present study was to investigate the effect of a period of endurance training along with electrical stimulation on the gene expression of some sex markers in testicular tissue of fasted obese rats.
Methods: In this experimental study, with a control group of 40 male Wistar rats (8 weeks old, weighing 200±19 grams), after induction of obesity and weight gain, randomly divided into 5 groups of 8: control, fasting-obese, fasting-endurance exercise, fasting-electrical stimulation and Fasting-endurance training-electrical stimulation were divided. Intervention groups for a period of 4 weeks under endurance sports activity (with a speed of 10 to 20 m/min and duration of 20 to 40 minutes), electrical stimulation (foot shock device for 0.5 mA and 20 minutes) and fasting (8 to 16 hours) were placed. After exercise and anesthesia, tissue sampling was done and after molecular processes, gene expression was measured using Real time-PCR machine. For data analysis, two-way analysis of variance test was used at a significance level of P< 0.05 and GraphPad software.
Results: The results showed that endurance training led to a significant increase in the expression of 5αR and aromatase genes in fasting obese rats compared to the obese group (P= 0.0001). Also, electrical stimulation and its combination with endurance training led to a significant increase in the expression of 5αR gene (P= 0.0001), but this increase was not significant in Aromatase gene (P= 0.377).
Conclusion: It seems that endurance training and electrical stimulation during fasting by increasing the expression of 5-alpha reductase and aromatase genes in obese samples is effective in their sexual health and leads to its improvement. Also, a combination of electrical stimulation and exercise can have positive effects on this process, but more studies are needed.
Methods: In this experimental study, with a control group of 40 male Wistar rats (8 weeks old, weighing 200±19 grams), after induction of obesity and weight gain, randomly divided into 5 groups of 8: control, fasting-obese, fasting-endurance exercise, fasting-electrical stimulation and Fasting-endurance training-electrical stimulation were divided. Intervention groups for a period of 4 weeks under endurance sports activity (with a speed of 10 to 20 m/min and duration of 20 to 40 minutes), electrical stimulation (foot shock device for 0.5 mA and 20 minutes) and fasting (8 to 16 hours) were placed. After exercise and anesthesia, tissue sampling was done and after molecular processes, gene expression was measured using Real time-PCR machine. For data analysis, two-way analysis of variance test was used at a significance level of P< 0.05 and GraphPad software.
Results: The results showed that endurance training led to a significant increase in the expression of 5αR and aromatase genes in fasting obese rats compared to the obese group (P= 0.0001). Also, electrical stimulation and its combination with endurance training led to a significant increase in the expression of 5αR gene (P= 0.0001), but this increase was not significant in Aromatase gene (P= 0.377).
Conclusion: It seems that endurance training and electrical stimulation during fasting by increasing the expression of 5-alpha reductase and aromatase genes in obese samples is effective in their sexual health and leads to its improvement. Also, a combination of electrical stimulation and exercise can have positive effects on this process, but more studies are needed.
Keywords: Obesity, Fasting, Endurance Exercise, Electrical Stimulation, 5-alpha reductase, Aromatase
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