Volume 21, Issue 4 (10-2021)
ijdld 2021, 21(4): 250-263 |
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Saie E, Mahmoudi F, Nourizadeh ٍ, Mahmoudi F. Evaluation of the Gene Expression of Tumor Necrotic Factor Alpha and Interlukine-6 in Rat Model of Diabetes Type 2 Treated with Silver Nanoparticles Synthetized by Galega Officinalis Extract. ijdld 2021; 21 (4) :250-263
URL: http://ijdld.tums.ac.ir/article-1-6079-en.html
URL: http://ijdld.tums.ac.ir/article-1-6079-en.html
1- Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran
2- Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran ,f.mahmoudi@uma.ac.ir
3- Depertment of Chemistry, Shahid Beheshti University, Tehran, Iran
2- Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran ,
3- Depertment of Chemistry, Shahid Beheshti University, Tehran, Iran
Abstract: (1339 Views)
Background: Hypoglycaemic effects of Galega officinalis and silver nanoparticles are established. In the present study, the effects of silver nanoparticles synthetized by Galega officinalis extract were investigated on gene expression of TNF-α, IL-6 and serum levels of liver enzymes in diabetes type 2.
Methods: In the present study 20 male Wistar rats in 4 group(n= 5 in each group) weighing 180- 200 gr were used. Control or nikotinamid and stroptozotosin induced diabetic rats receieved intraperitoneal injection of saline or 2/5mg/Kg silver nanoparticles synthetized by Galega officinalis extract or chemichal method for 14 days respectively. One day after the last injections, serum samples and adipose tissue were collected. Mean serum concentration of glucose, urea, creatinine, alanine amino transferase(ALT), and aspartate amino transferase(AST) were determined by spectrophotometry. Mean relative gene expression of TNF-α and IL-6 were determined by method of real time PCR.
Results: Mean serum levels of ALT and AST enzymes, glucose, urea significantly decreased in diabetic rats receiving chemichal or green silver naoparticles compared to diabetic group. Mean relative gene expression of TNF-α and IL-6 significantly decreased in diabetic rats receiving chemichal or green silver naoparticles compared to diabetic ones.
Conclusion: Both chemical and green synthetized silver nanoparticles may prevent hepatocyes damages and they may improve insulin resistance in diabetes type 2 partly via decresing pro- inflammatory factors.
Methods: In the present study 20 male Wistar rats in 4 group(n= 5 in each group) weighing 180- 200 gr were used. Control or nikotinamid and stroptozotosin induced diabetic rats receieved intraperitoneal injection of saline or 2/5mg/Kg silver nanoparticles synthetized by Galega officinalis extract or chemichal method for 14 days respectively. One day after the last injections, serum samples and adipose tissue were collected. Mean serum concentration of glucose, urea, creatinine, alanine amino transferase(ALT), and aspartate amino transferase(AST) were determined by spectrophotometry. Mean relative gene expression of TNF-α and IL-6 were determined by method of real time PCR.
Results: Mean serum levels of ALT and AST enzymes, glucose, urea significantly decreased in diabetic rats receiving chemichal or green silver naoparticles compared to diabetic group. Mean relative gene expression of TNF-α and IL-6 significantly decreased in diabetic rats receiving chemichal or green silver naoparticles compared to diabetic ones.
Conclusion: Both chemical and green synthetized silver nanoparticles may prevent hepatocyes damages and they may improve insulin resistance in diabetes type 2 partly via decresing pro- inflammatory factors.
Type of Study: Research |
Subject:
Special
Received: 2021/08/5 | Accepted: 2021/10/25 | Published: 2021/10/2
Received: 2021/08/5 | Accepted: 2021/10/25 | Published: 2021/10/2
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