Volume 19, Issue 2 (1-2020)
ijdld 2020, 19(2): 61-70 |
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Soltanmohammadi F, Mohsenzadeh M, Feizollahi F. THE EFFECT OF 8 WEEKS OF HIIT TRAINING AND SUPPLEMENTATION OF BLACK GRAPE SEED EXTRACT ON WNT AND Β-CATENIN GENE EXPRESSION IN PANCREATIC TISSUE IN MALE RATS WITH TYPE 2 DIABETES.. ijdld 2020; 19 (2) :61-70
URL: http://ijdld.tums.ac.ir/article-1-5909-en.html
URL: http://ijdld.tums.ac.ir/article-1-5909-en.html
1- Department of Physical Education and Sport Sciences, Islamic Azad University, Branch Karaj, Alborz, Iran
2- Department of Physical Education and Sport Sciences, Islamic Azad University, Branch Karaj, Alborz, Iran ,m.mohsenzadeh@kiau.ac.ir
2- Department of Physical Education and Sport Sciences, Islamic Azad University, Branch Karaj, Alborz, Iran ,
Abstract: (2987 Views)
Background: The aim of the present study was to investigate the effect of 8 weeks of HIIT training and supplementation of black grape seed extract on Wnt and Β-catenin gene expression in pancreatic tissue in male rats with type 2 diabetes.
Methods: In this experimental study, 40 male Wistar rats, with average weight of 250 ± 20 gr, after induction of diabetes by STZ, were randomly divided into 5 groups: training- supplement, training-placebo, supplement and diabetic-control and base control. After becoming diabetic, rats were familiarized with protocol for one week and then trained for eight weeks, five days a week. They ran on treadmill with 90% of VO2 max. Intragastric injection of GSPE extracted from black grape seed (Cabernet sauvignon) once a day for 8 weeks (250 mg/kg, proanthocyanidin content >96%). 24 hours after the last session of training, pancreas tissue was extracted, and the level of gene expression was measured by Real Time-PCR. In all statistical analyzes, the statistical significance level was considered to be 0.05.
Results: Eight weeks and supplement significantly increased the mean expression of Wnt and Β-catenin genes in pancreatic tissue of type 2 diabetic rats but the effect of exercise with black grape seed extract on the expression of Wnt genes in the pancreas of diabetic rats was not significant.
Conclusion: It seems that regulating the expression of Wnt and Β-catenin genes through exercise and consumption of black grape seed extract is likely to improve and maintain the function of pancreatic beta cells, especially in diabetic samples.
Methods: In this experimental study, 40 male Wistar rats, with average weight of 250 ± 20 gr, after induction of diabetes by STZ, were randomly divided into 5 groups: training- supplement, training-placebo, supplement and diabetic-control and base control. After becoming diabetic, rats were familiarized with protocol for one week and then trained for eight weeks, five days a week. They ran on treadmill with 90% of VO2 max. Intragastric injection of GSPE extracted from black grape seed (Cabernet sauvignon) once a day for 8 weeks (250 mg/kg, proanthocyanidin content >96%). 24 hours after the last session of training, pancreas tissue was extracted, and the level of gene expression was measured by Real Time-PCR. In all statistical analyzes, the statistical significance level was considered to be 0.05.
Results: Eight weeks and supplement significantly increased the mean expression of Wnt and Β-catenin genes in pancreatic tissue of type 2 diabetic rats but the effect of exercise with black grape seed extract on the expression of Wnt genes in the pancreas of diabetic rats was not significant.
Conclusion: It seems that regulating the expression of Wnt and Β-catenin genes through exercise and consumption of black grape seed extract is likely to improve and maintain the function of pancreatic beta cells, especially in diabetic samples.
Type of Study: Research |
Subject:
Special
Received: 2019/12/14 | Accepted: 2020/02/12 | Published: 2020/02/29
Received: 2019/12/14 | Accepted: 2020/02/12 | Published: 2020/02/29
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